HPLC Method for Analysis of Dichloran on Obelisc R Column on Alltesta™

HPLC Method for Dichloran on Obelisc R by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dichloran 

Dichloran, also known as 2,6-dichloro-4-nitroaniline, is a herbicide with the chemical formula C6H4Cl2N2O2. It is used primarily as a pre- and post-harvest gerbicide on various plants including but not limited to peaches, grapes, lettuce, celery, and more. It works through inhibiting hyphal growth, which delays fungal spore germination. You can find detailed UV spectra of Dichloran and information about its various lambda maxima by visiting the following link.

Dichloran can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnObelisc R, 2.1 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN -50/50%
BufferAmFm pH 3.0 – 20 mM
Flow Rate0.3 mL/min
DetectionUV 275 nm
Class of CompoundsHerbicide
Analyzing CompoundsDichloran

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Dichloran
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.