HPLC Method for Analysis of Cysteine on Primesep 100 Column

HPLC Method for Analysis of Cysteine on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine.

L-Cysteine is an amino acid with the chemical formula C5H10N2O3. It is primarily a building block for protein, but it also has antioxidant effects. On occasion, it is used to support people dealing with cancer, diabetes, and hangover, but there is yet to be substantial evidence that it works. Poultry, egg, beef, and whole grains are rich sources of the amino acid. You can find detailed UV spectra of Cysteine and information about its various lambda maxima by visiting the following link.

Cysteine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 192nm
Limit Of Detection*4 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAmino Acid
Analyzing CompoundsCysteine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cysteine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.