HPLC Method for Analysis of Creatine, Creatinine on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Creatine, Creatinine
Creatine is a naturally occurring compound found in muscles and is used to produce energy during high-intensity exercise. It is synthesized in the body from amino acids and stored in muscles for quick energy release. Creatinine, on the other hand, is a waste product produced from the breakdown of creatine. It is filtered by the kidneys and excreted in urine. The measurement of creatinine levels in the blood and urine is commonly used to assess kidney function, as high levels may indicate impaired kidney function or other health issues.
Creatine and Creatinine can be analyzed and separated using a Primesep 200 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid as a buffer. Detection is carried out using UV.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Sample | 1. 0.2 mg/mL, 2. 0.3 mg/mL |
| Injection volume | 5 µl |
| LOD* | 1. 46.5 ppb, 2. 27.4 ppb |
| Class of Compounds | Quaternity amines |
| Analyzing Compounds | Creatine, Creatinine |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Creatinine
