HPLC Method for Chlorogenic acid on Chromni™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorogenic acid
Chlorogenic acid is a natural compound that belongs to the polyphenol family, which is abundant in various plants. It is particularly prevalent in coffee beans, fruits, vegetables, and whole grains. One of its primary dietary sources is coffee, where it is found in significant amounts. It is well known for it’s antioxidant properties, which means it can help neutralize free radicals in the body. Free radicals are molecules that can damage cells and contribute to aging and various diseases. Evidence suggests that chlorogenic acid may have a positive impact on blood sugar levels. It may help regulate glucose metabolism, making it of interest in managing diabetes and insulin resistance. You can find detailed UV spectra of Chlorogenic acid and information about its various lambda maxima by visiting the following link.
Chlorogenic acid can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
| Column | Chromni™, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 80% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Chlorogenic acid |
Application Column
Chromni™
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
