HPLC Method for Analysis of Butylated Hydroxyanisole (BHA) on Newcrom R1 Column on Cromite™

HPLC Method for Butylated Hydroxyanisole (BHA) on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Butylated Hydroxyanisole (BHA)

Butylated Hydroxyanisole (BHA) is a petrochemical with the chemical formula C11H16O2. Due to it’s antioxidant properties, it is used in preservation of food, cosmetics, and pharmaceuticals. Due to previous experiments in animals, it is suspected to be a carcinogen for humans.

Butylated Hydroxyanisole (BHA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate0.5 mL/min
DetectionUV 275 nm
Class of CompoundsPhenolic Antioxidants
Analyzing CompoundsButylated Hydroxyanisole (BHA)

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Butylated Hydroxyanisole (BHA)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.