HPLC Method for Analysis of Biopterin on Primesep 100 Column on Alltesta™

HPLC Method for Biopterin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin 

Biopterin is a petrin derivative with the chemical formula C9H11N5O3. As a cofactor for aromatic amino acid hydroxylases, it is a part of the synthesis of neurotransmitters like dopamine, serotonin, and norepinephrine. A biopterin imbalance can lead to a variety of disorders, most of which lead to neurological problems.

Biopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsOrganic Compound
Analyzing CompoundsBiopterin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Biopterin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.