HPLC Method for Analysis of Benzoic Acid on Newcrom BH Column on Alltesta™

HPLC Method for Benzoic Acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoic Acid 

Benzoic Acid is an organic acid with antimicrobial properties.  It is typically used in cosmetics, food, and pharmaceuticals as a preservative against yeast and mold. As treatment, it is also used as a topical medicine of fungal skin infections. It can be naturally found in many berries and vegetable resins. With it’s chemical formula being C7H6O2, it is considered the simplest aromatic carboxylic acid. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.

Benzoic Acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnNewcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 80%
BufferSulfuric Acid
Flow Rate0.5 mL/min
DetectionUV 275 nm
Class of CompoundsAcid
Analyzing CompoundsBenzoic Acid

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzoic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.