HPLC Method for Analysis of Acetylsalicylic acid (ASA) on Primesep 100 Column on Alltesta™

HPLC Method for Acetylsalicylic Acid (Aspirin) on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetylsalicylic Acid (Aspirin)

Acetylsalicylic acid (ASA), also known as Aspirin, is an organic compound with the molecular formula C9H8O4. It is a nonsteroidal anti-inflammatory drug (NSAID) that is used to reduce fever, pain, and inflammation. It is also used to treat Kawasaki disease, pericarditis, and rheumatic fever. Aspirin is a genetic medication that is widely available and can be procured without a prescription. It is one of the oldest NSAIDs in the world.

Acetylsalicylic Acid (Aspirin) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsDrugs
Analyzing CompoundsAcetylsalicylic Acid (Aspirin)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetylsalicylic Acid (Aspirin)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.