HPLC Method for Analysis of Ascorbic Acid on Newcrom BH Column

HPLC Method for Ascorbic Acid on Newcrom BH by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Ascorbic Acid

Ascorbic Acid is a vitamin with the molecular formula C6H8O6. Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus.

Ascorbic Acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferAcetic Acid
Flow Rate1.0 ml/min
DetectionUV 250
LOD* UV25ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsVitamin
Analyzing CompoundsAscorbic Acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ascorbic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.