HPLC Method for Analysis of Aconitic Acid on Chromni Column

HPLC Method for Analysis of Aconitic Acid on BIST A Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Aconitic acid, trans-Aconitic acid.

Aconitic acid is an organic compound with the chemical formula C6H6O6. It is primarily used to create nutty flavors in foods. It is also a common intermediate in the Kreb’s cycle when Citrate is changed into D-isocitrate. You can find detailed UV spectra of Aconitic acid, trans-Aconitic acid and information about its various lambda maxima by visiting the following link.

Aconitic acid, trans-Aconitic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition

ColumnChromni, 2.1 x 100 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN – 75/25%
BufferAmmonium acetate pH 5.0 – 210 mM
Flow Rate0.2 ml/min
DetectionESI-SIM: [M-H]1- 173, UV 230
Limit Of Detection*LC MS 100 ppb, UV 28 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAcid, Carboxylic acids, Tricarboxylic acids
Analyzing CompoundsAconitic acid, trans-Aconitic acid

Application Column

Chromni

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Aconitic acid
trans-Aconitic acid
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.