HPLC Method for Analysis of Acesulfame K on Newcrom BH Column

HPLC Method for Analysis of Acesulfame K on Newcrom BH Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acesulfame K.

Acesulfame K, also known as Acesulfame potassium and Ace K, is a sugar substitute with the formula C4H4KNO4S. It is significantly sweeter than other popular sugars, but has a bitter aftertaste at high concentration. You can find detailed UV spectra of Acesulfame K and information about its various lambda maxima by visiting the following link.

Acesulfame K can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and sulfuric acid. Detection is performed using UV.

Condition

ColumnNewcrom BH, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 192 nm
LOD*3 ppb
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsSugar
Analyzing CompoundsAcesulfame K

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acesulfame K
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.