HPLC Method for Separation of 2-Dodecylphenol and 4-Dodecylphenol on Chromni Column

HPLC Method for Analysis of 2-Dodecylphenol, 4-Dodecylphenol on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Dodecylphenol, 4-Dodecylphenol.

Dodecylphenol is an alkylphenol chemical compound with the formula C18H30O. It is used primarily in industrial manufacturing of detergents, lubricants, and fuels. It is used as an intermediate in the mining industry as well as in the production of a wide variety of resins.

2-Dodecylphenol, 4-Dodecylphenol can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN
BufferNone
Flow Rate1.0 ml/min
DetectionUV 220 nm
LOD*1. 34.5 ppb, 2. 35.2 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsPhenol
Analyzing Compounds2-Dodecylphenol, 4-Dodecylphenol

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2-Dodecylphenol
4-Dodecylphenol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.