HPLC Method for 2-Aminopyridine on Chromni™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Aminopyridine
2-Aminopyridine is an synthetic organochlorine compound with the molecular formula C5H6N2.
Properties:
Appearance: Typically a white powder or crystals.
Molecular weight: ~94.11 g/mol
Solubility: Soluble in water and alcohol.
Uses: Production of the drugs tenoxicam, sulfapyridine, piroxicam, tripelennamine.
2-Aminopyridine can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate. Detection is performed using UV.
Column | Chromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
Mobile Phase | MeCN – 70% |
Buffer | Ammonium Formate – 10mM |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Class of Compounds | Tertiary Amine |
Analyzing Compounds | 2-Aminopyridine |
Application Column
Chromni™
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended