HPLC Method for Evogliptin on Primesep B by SIELC Technologies

Evogliptin is a drug that belongs to the class of dipeptidyl peptidase-4 (DPP-4) inhibitors and has the chemical formula C₁₉H₂₆F₃N₃O₃. It is used primarily for the treatment of type 2 diabetes mellitus. DPP-4 inhibitors work by blocking the action of the enzyme DPP-4, which in turn increases the levels of incretin hormones such as GLP-1 (glucagon-like peptide-1). This increase helps to stimulate insulin release and suppress glucagon secretion, thereby helping to regulate blood sugar levels in patients with diabetes. You can find detailed UV spectra of Evogliptin and information about its various lambda maxima by visiting the following link.

Evogliptin can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 200 nm