HPLC Method for Analysis of Clonidine on Primesep B by SIELC Technologies
Clonidine is a medication with the chemical formula C9H9Cl2N3. It is primarily prescribed for the treatment of hypertension, especially in cases where other antihypertensive medications have not been effective or well-tolerated. Clonidine is available in various formulations, including tablets, patches, and oral suspension.
Clonidine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 290 nm
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN/H2O – 40-80%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| Class of Compounds | Sulfonamides, sulphonamides, loop diuretics |
| Analyzing Compounds | Clonidine |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
ClonidineApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
