HPLC Method for Analysis of Abacavir on Primesep 100 Column

HPLC Method for Abacavir on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Abacavir on Primesep 100 Column by SIELC Technologies

Abacavir, often written as ABC, is a medication with the formula C14H18N6O. It is used primarily as a part of HIV/AIDS treatments. As a nucleoside reverse transcriptase inhibitor and a guanosine, it works through inhibiting viral replication. It is in the World Health Organization’s List of Essential Medicines. You can find detailed UV spectra of Abacavir and information about its various lambda maxima by visiting the following link.

Abacavir can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and H2SO4 as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferH2SO4  – 0.1%
Flow Rate1 ml/min
DetectionUV 296 nm
LOD*2 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsMedication
Analyzing CompoundsAbacavir

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Abacavir
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.