HPLC Method for Tylosin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tylosin
Tylosin is an antibiotic with the molecular formula C46H77NO17. It is typically used to treat infections and inflammations, especially in veterinary cases. It can be administered orally as a powder or liquid or through injection.
Tylosin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using ELSD.
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | Gradient MeCN/H2O from 40/60% to 80/20% 10 min |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 286 |
Class of Compounds | Antibiotic |
Analyzing Compounds | Tylosin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended