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Xanthohumol (XN) is a flavonoid compound found in beer that contributes to its flavor and bitterness. There have also been interest in flavonoids and their possible medical applications. Studies have shown that it has anticancer activity and the ability to lower cholesterol and blood sugar. XN can be retained in HPLC on a Newcrom AH mixed-mode column with a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) as buffer and UV detected at 370nm.
Newcrom AH, 4.6x150 mm, 5 µm, 100A
MeOH/H2O - 70/30%
H2SO4 - 0.1%
UV 370 nm
Class of Compounds
Hydrophobic, Acid, Chalconoid
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