HPLC Determination of Xanthohumol on Newcrom AH Column

HPLC.cloud View on hplc.cloud Xanthohumol (XN) is a flavonoid compound found in beer that contributes to its flavor and bitterness. There have also been interest in flavonoids and their possible medical applications. Studies have shown that it has anticancer activity and the ability to lower cholesterol and blood sugar. XN can be retained in HPLC on a Newcrom AH mixed-mode column with a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) as buffer and UV detected at 370nm.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 70/30%
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV 370 nm
 

Description

Class of Compounds Hydrophobic, Acid, Chalconoid
Analyzing Compounds Xanthohumol
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Application Analytes:
Xanthohumol