HPLC Determination of Uridine Diphosphate Galactose on Newcrom B Column

HPLC Method for Uridine 5'-diphosphogalactose disodium salt on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine 5'-diphosphogalactose disodium salt.

Uridine Diphosphate Galactose (UDP-galactose) is an intermediary formed in the biosynthesis of polysaccharides with the chemical formula C15H24N2O17P2. It is important in glycolysis, which is a metabolic pathway that converts glucose into pyruvate.

UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.

Condition
Column Newcrom B, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm – pH 3.0 – 20 mm
Flow Rate 1.0 ml/min
Detection CAD, 262 nm

 

Description
Class of Compounds
 Sugar Phosphate
Analyzing Compounds Uridine 5'-diphosphogalactose disodium salt

 

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Uridine 5′-diphosphogalactose disodium salt
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.