HPLC Determination of Uridine Diphosphate Galactose on Newcrom B Column

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine Diphosphate Galactose









Uridine Diphosphate Galactose (UDP-galactose) is an intermediary formed in the biosynthesis of polysaccharides.
UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.




Condition
Column Newcrom B, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm – pH 3.0 – 20 mm
Flow Rate 1.0 ml/min
Detection CAD, 262 nm

 

Description
Class of Compounds
 Sugar Phosphate
Analyzing Compounds Sodium, Uridine Diphospate Galactose

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Uridine 5′-diphosphogalactose disodium salt
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.