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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Uridine 5'-diphosphogalactose disodium salt on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine 5'-diphosphogalactose disodium salt.

Uridine Diphosphate Galactose (UDP-galactose) is an intermediary formed in the biosynthesis of polysaccharides with the chemical formula C₁₅H₂₄N₂O₁₇P₂. It is important in glycolysis, which is a metabolic pathway that converts glucose into pyruvate.

UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.

HPLC Method for Uridine-5'-diphosphate-glucose, Uridine 5'-diphosphogalactose disodium salt, Uridine 5′-diphospho-N-acetylgalactosamine on Primesep N by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Gadolinium-DOTA

Uridine sugar diphospates are a class of complex nucleotide sugars that are integral to metabolic processes in the cell. Uridine diphosphate galactose (UDP-Galactose), Uridine diphosphate glucose (UDP-Glucose), and uridine diphosphate N-acetylglucosamine (UDP-N-acetylgalactoseamine or UDP-GlcNAc) are some of the most well-known nucleotide sugars.

Uridine Diphosphate Glucose (UDP-glucose) is an an important nucleotide sugar with the chemical formula C₁₅H₂₄N₂O₁₇P₂. It is involved in metabolic processes in the cell. It is a precursor to glycogen, UDP-galactose, and UDP-glucuronic acid, as well as other polysacchrides and glycosphingolipids.

Uridine Diphosphate Galactose (UDP-galactose) is an intermediary formed in the biosynthesis of polysaccharides with the chemical formula C₁₅H₂₄N₂O₁₇P₂. It is important in glycolysis, which is a metabolic pathway that converts glucose into pyruvate.

These three uridine sugar diphosphate compounds can be detected in the low UV regime. Using a Primesep N normal-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose, UDP-glucose, and UDP-GIcNAc can be retained, separated, and analyzed. This analysis method can be UV detected at 260 nm.