| CAS Number | 28053-08-9 |
|---|---|
| Molecular Formula | C15H22N2Na2O17P2 |
| Molecular Weight | 610.27 |
| InChI Key | PKJQEQVCYGYYMM-OUJOOSCPSA-L |
| Synonyms |
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Applications:
HPLC Determination of Uridine Diphosphate Galactose on Newcrom B Column
May 13, 2021
Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine Diphosphate Galactose
Uridine Diphosphate Galactose (UDP-galactose) is an intermediary formed in the biosynthesis of polysaccharides.
UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.
| Column | Newcrom B, 4.6×150 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm – pH 3.0 – 20 mm |
| Flow Rate | 1.0 ml/min |
| Detection | CAD, 262 nm |
| Class of Compounds |
Sugar Phosphate |
| Analyzing Compounds | Sodium, Uridine Diphospate Galactose |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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HPLC Separation of Uridine Sugar Diphospates on Primesep N Column
May 11, 2021
High Performance Liquid Chromatography (HPLC) Method for Analysis of Gadolinium-DOTA
Uridine sugar diphospates are a class of complex nucleotide sugars that are integral to metabolic processes in the cell. Uridine diphosphate galactose (UDP-Galactose), Uridine diphosphate glucose (UDP-Glucose), and uridine diphosphate N-acetylglucosamine (UDP-N-acetylgalactoseamine or UDP-GlcNAc) are some of the most well-known nucleotide sugars.
These three uridine sugar diphosphate compounds can be detected in the low UV regime. Using a Primesep N normal-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose, UDP-glucose, and UDP-GIcNAc can be retained, separated, and analyzed. This analysis method can be UV detected at 260 nm.
| Column | Primesep N, 4.6×250 mm, 3 µm, 100A |
| Mobile Phase | MeCN – 80% |
| Buffer | Ammonium formate pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Class of Compounds | Sugar, Phospate |
| Analyzing Compounds | Uridine-5′-diphosphate-glucose, Uridine 5′-diphospho-N-acetylgalactosamine disodium salt, Uridine 5′-diphosphogalactose disodium salt |
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsUridine 5′-diphospho-N-acetylgalactosamine
Uridine-5′-diphosphate-glucose
