HPLC Determination of Thiocyanate on Newcrom BH Column

HPLC Method for Potassium Thiocyanate on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Potassium Thiocyanate.

Thiocyanate is a pseudohalide with the chemical formula [SCN]−. It works as a biological metabolite in humans and is produced in the liver during detoxification process. While it is a part of the body’s antimicrobial defenses, high presence of it can hinder iodine uptake, leading to an iodine deficiency.

Potassium Thiocyanate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate buffer. Detection is performed using UV.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer Ammonium Acetate pH 5.0 50 mM
Flow Rate 1.0 ml/min
Detection UV 235 nm, MS-compatible mobile phase

Description
Class of Compounds Anion
Analyzing Compounds Potassium Thiocyanate

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Potassium Thiocyanate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.