HPLC Method for Sulfosuccinic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfosuccinic Acid.
Sulfosuccinic acid is a surfactant with the chemical formula C4H6O7S. It is used as a dispersing agent in pesticides, herbicides, and pigments. In fertilizers, it is used as a complexing agent. It is also used to enhance solubility and bioavailability of drugs in oral dosages.
Sulfosuccinic acid can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Column | Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O -10/90% |
Buffer | H2SO4 – 0.3% |
Flow Rate | 0.5 ml/min |
Detection | UV, 200 nm |
Class of Compounds |
Acid |
Analyzing Compounds | Sulfosuccinic acid |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended