HPLC Determination of Sulfosuccinic Acid on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfosuccinic Acid.

Sulfosuccinic acid is a surfactant used in cosmetics and pharmaceuticals. Sulfosuccinic acid can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.

Condition
Column Newcrom BH, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN/H2O -10/90%
Buffer  H2SO4 – 0.3%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Acid
Analyzing Compounds Sulfosuccinic Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Sulfosuccinic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.