HPLC Determination of Quercetin on Primesep D column

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method of Quercetin.

Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, apples, berries, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. Quercetin can be retained in HPLC using a Primesep D mixed-mode column using a mobile phase of acetonitrile (ACN), water and Trifluoroacetic acid (TFA) buffer. Detection can be achieved with UV 270 nm , mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).



Condition 1
Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 40%
Buffer TFA – 0.05%
Flow Rate 1.0 ml/min
Detection 270 nm

Description
Class of Compounds Phenolics compound, Flavonoid
Analyzing Compounds  Quercetin

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Quercetin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.