HPLC Determination of Quercetin on Primesep D Column

Method for Quercetin on Primesep D by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method of Quercetin.

Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, apples, berries, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. It has the chemical formula C15H10O7.

Quercetin can be retained in HPLC using a Primesep D mixed-mode column using a mobile phase of acetonitrile (ACN), water and Trifluoroacetic acid (TFA) buffer. Detection can be achieved with UV 270 nm , mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).

Condition 1
Column Primesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 40%
Buffer TFA – 0.05%
Flow Rate 1.0 ml/min
Detection 270 nm

Description
Class of Compounds Phenolics compound, Flavonoid
Analyzing Compounds Quercetin

 

Application Column

Primesep D

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Quercetin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.