HPLC Method for Octylphosphonic acid on Newcrom A by SIELC Technologies
Octylphosphonic acid (OPA) is a surfactant with the chemical formula C8H19O3P. It can be used to synthesize self-assembled monolayers (SAMs), polymeric matrices, and as a coating for super-resolution microscopy.
Using a Newcrom A mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer, OPA can be retained, measured, and analyzed. This analysis method can be used in conjunction with charged aerosol detection (CAD).
| Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Hydrophobic, Ionizable |
| Analyzing Compounds | Octylphosphonic acid |
Application Column
Newcrom AH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Octylphosphonic acidApplication Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
