HPLC Determination of Octylphosphonic acid on Newcrom A

Separation type: Liquid Chromatography Mixed-mode




HPLC.cloud
View on hplc.cloud









High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC



Octylphosphonic acid (OPA) is a surfactant that can be used to synthesize self-assembled monolayers (SAMs), polymeric matrices, and as a coating for super-resolution microscopy.
Using a Newcrom A mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer, OPA can be retained, measured, and analyzed. This analysis method can be used in conjunction with charged aerosol detection (CAD).




Condition
Column Newcrom A, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer Formic Acid – 0.5%
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
 Hydrophobic, Ionizable
Analyzing Compounds  Octylphosphonic acid

 

Application Column

Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Octylphosphonic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.