HPLC Determination of Fipronil on Newcrom R1 Column

HPLC Method for Fipronil on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fipronil.

Fipronil is an insecticide with the chemical formula C12H4Cl2F6N4OS. It works through causing hyperexcitation of the nerves and muscles in contaminated insects.  In Texas, it is often used to control invasive species of ants and wasps. It is also an active ingredient in flea and tick treatments for pets. WHO classifies it as a Class II, moderately hazardous, pesticide.

Fipronil can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) without a buffer. Detection is performed using UV.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O
Buffer No
Flow Rate 1.0 ml/min
Detection UV 200 nm (MS-compatible mobile phase)

Description
Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Fipronil

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Fipronil
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.