HPLC Method for Analysis of Cypermethrin on Primesep 200 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Cypermethrin is a synthetic pyrethroid with neurotoxic properties that is typically used as an insecticide. Cypermethrin can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Formic acid (FA) ionic modifier. This analysis method can be detected in the low UV regime at 270 nm as well as with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cypermethrin
Condition
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 60-80%, 10 min |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm, ELSD, 50C |
| Peak Retention Time | 7.21 |
Description
| Class of Compounds | Pesticide |
| Analyzing Compounds | Cypermethrin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Cypermethrin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
