HPLC Method for Analysis of Cypermethrin on Primesep 200 Column

HPLC Method for Analysis of Cypermethrin on Primesep 200 by SIELC Technologies

HPLC Method for Analysis of Cypermethrin on Primesep 200 Column by SIELC Technologies
HPLC Method for Analysis of Cypermethrin on Primesep 200 by SIELC Technologies

Cypermethrin is a synthetic pyrethroid with the chemical formula C22H19Cl2NO3. It is primarily used as an insecticide, working effectively due to its neurotoxic properties. It is widely used due to degrading easily in soil. Despite that, it can be effective for weeks.

Cypermethrin can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Formic acid (FA) ionic modifier. This analysis method can be detected in the low UV regime at 270 nm as well as with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

Condition

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 60-80%, 10 min
BufferFormic Acid – 0.5%
Flow Rate1.0 ml/min
DetectionUV 270 nm, ELSD, 50C
Peak Retention Time7.21

Description

Class of CompoundsPesticide
Analyzing CompoundsCypermethrin

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cypermethrin
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.