HPLC Determination of Cefepime on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 


HPLC.cloud
View on hplc.cloud

 

High Performance Liquid Chromatography (HPLC) Method for Analysis Cefepime

Cefepime is a fourth generation cephalosporin antibiotic usually reserved for treatment of severe nosocomial pneumonia and febrile neutropenia as well as multiple drug-resistant microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Cefepime can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.

Condition
Column Primesep 100,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 – 0.5%
Flow Rate 1.0  ml/min
Detection UV 270 nm

 

Description
Class of Compounds
  Drug
Analyzing Compounds Cefepime

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cefepime
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.