HPLC Method for Analysis of Bromhexine on Primesep 200 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromhexine on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Bromhexine on Primesep 200 Column
HPLC Method for Analysis of Bromhexine on Primesep 200 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromhexine

Condition

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 60/40%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210, 245, 315 nm
Peak Retention Time2.81, 4.22 min

Description

Class of CompoundsDrugs
Analyzing CompoundsBromhexine
Application Analytes:
Bromhexine
Bromhexine hydrochloride

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.