HPLC Method for Analysis of Bromhexine on Primesep 200 Column

HPLC Method for Bromhexine, Bromhexine hydrochloride on Primesep 200 by SIELC Technologies

HPLC Method for Analysis of Bromhexine on Primesep 200 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromhexine, Bromhexine hydrochloride.

Bromhexine is a popular mucolytic with the chemical formula C14H20Br2N2. It is used to treat excessive mucus found in the respiratory system from a variety of illnesses. It works through increasing the production of serous mucus, which thins phlegm. This helps transport phlegm out of the upper respiratory tract. You can find detailed UV spectra of Bromhexine and information about its various lambda maxima by visiting the following link.

Bromhexine, Bromhexine hydrochloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 60/40%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210, 245, 315 nm
Peak Retention Time2.81, 4.22 min

Description

Class of CompoundsDrugs
Analyzing CompoundsBromhexine, Bromhexine hydrochloride

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bromhexine
Bromhexine hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.