HPLC Method for 5-Hydroxymethyl-2-furaldehyde (5-HMF) on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 5-Hydroxymethyl-2-furaldehyde (5-HMF).
5-Hydroxymethyl-2-furaldehyde (HMF), also known as 5-(Hydroxymethyl)furfural, is an organic compound derived from sugars and is of interest due to its potential as a platform chemical for biofuels and biochemicals.
HMF plays a notable role in the flavor and aroma development of whisky. Understanding its formation, impact, and concentration can provide insights into the aging process and overall quality of the whisky, contributing to the appreciation and enjoyment of this complex beverage.
Furan is a chemical contaminant that forms in some foods during traditional heat treatment techniques, such as cooking, jarring, and canning.
5-Hydroxymethyl-2-furaldehyde (5-HMF) can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 210 and 275 nm.
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | 284 nm |
| Class of Compounds | Hydrophilic |
| Analyzing Compounds | 5-Hydroxymethyl-2-furaldehyde (5-HMF) |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
