High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Methoxypyrazineon Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Methoxypyrazines are an important class of odorous, flavored compounds that can greatly affect the taste of wines. In particular with respect to white wines, they are highly associated with vegetal and herbaceous aromas and flavors; these are often referred to as green characteristics of the wine. Thus winemakers tend to try to minimize the Methoxypyrazine content in their wines; this can be done through extractment, exposure to oak barrels (or oak chips), or minimizing the amount of grape skins and and stems in the production process. 2-Methoxypyrazine can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Peak Retention Time | 3.92 min |
Description
| Class of Compounds | Pyrazines |
| Analyzing Compounds | 2-Methoxypyrazine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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