High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Methoxypyrazine on Primesep 100 by SIELC Technologies.
Methoxypyrazines are an important class of odorous, flavored compounds that can greatly affect the taste of wines. In particular with respect to white wines, they are highly associated with vegetal and herbaceous aromas and flavors; these are often referred to as green characteristics of the wine. Thus winemakers tend to try to minimize the Methoxypyrazine content in their wines; this can be done through extractment, exposure to oak barrels (or oak chips), or minimizing the amount of grape skins and and stems in the production process.
2-Methoxypyrazine can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Peak Retention Time | 3.92 min |
Description
| Class of Compounds | Pyrazines |
| Analyzing Compounds | 2-Methoxypyrazine] |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
