HPLC Chiral Separation of Uniconazole Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Uniconazole Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Uniconazole.

Uniconazole is a plant growth regulator with the chemical formula C15H18ClN3O. As a pesticide, works through inhibiting the production of gibberellins, which are plant growth hormones. It is typically used in perennials and ornamental plants to ensure they are a marketable size before they bloom. You can find detailed UV spectra of Uniconazole and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Uniconazole enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Uniconazole can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and Ethanol. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 250 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution1.57

Description

Class of CompoundsPesticide
Analyzing CompoundsUniconazole

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Uniconazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.