HPLC Chiral Separation of Trimipramine Enantiomers on Cyralis CM Column

HPLC Chiral Method for Analysis of Trimipramine, Trimipramine maleate Enantiomers on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimipramine, Trimipramine maleate.

Trimipramine is a tricyclic antidepressant (TCA) with the chemical formula C20H26N2. It is used primarily used to treat depression, but it has also been used to treat insomnia, anxiety disorders, and psychosis due to it’s sedative, anxiolytic, and antipsychotic effects. Unlike other TCAs, it is a weaker monoamine reuptake inhibitor. You can find detailed UV spectra of Trimipramine, Trimipramine maleate and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Trimipramine, Trimipramine maleate enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Trimipramine, Trimipramine maleate can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, reversed phase
Mobile PhaseNaClO4/MeCN
Flow Rate1.0 mL/min
DetectionUV 220 nm
Resolution1.51

Description

Class of CompoundsDrug
Analyzing CompoundsTrimipramine, Trimipramine maleate

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: reversed phase

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Application Analytes:
Trimipramine
Trimipramine maleate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.