HPLC Chiral Separation of Tebuconazole Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Tebuconazole Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tebuconazole.

Tebuconazole is a triazole fungicide with the chemical formula C16H22ClN3O. It is used against to plant pathogenic fungi. Despite the U.S. Food and Drug Administration considering this fungicide safe, it is considered a possible carcinogen with the rating of C. According to the World Health Organization, it has moderate toxicity and is slightly hazardous. You can find detailed UV spectra of Tebuconazole and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Tebuconazole enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Tebuconazole can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 250 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/DEA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution1.72

Description

Class of CompoundsFungicide
Analyzing CompoundsTebuconazole

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Tebuconazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.