HPLC Chiral Separation of Norephedrine Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Norephedrine Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Norephedrine.

Norephedrine, also known as Phenylpropanolamine, is a drug with the chemical formula C9H13NO. Iti s primarily used as a decongestant and appetite suppressant. It works as a selective norepinephrine and dopamine releasing agent. Despite being under investigation, it is not considered to be a performance-enhancing drug.

This application describes a normal-phase chiral HPLC method for the separation of Norephedrine enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Norephedrine can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and Ethanolamine. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/Ethanolamine
Flow Rate1.0 mL/min
DetectionUV 220 nm
Resolution2.73

Description

Class of CompoundsOragnic
Analyzing CompoundsNorephedrine

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Norephedrine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.