HPLC Chiral Separation of Mirtazapine Enantiomers on Cyralis CM Column

HPLC Chiral Method for Analysis of Mirtazapine Enantiomers on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mirtazapine.

Mirtazapine is an atypical tetracyclic antidepressant with the chemical formula C17H19N3. It is used primarily to treat depression that is complicated by anxiety or insomnia.

This application describes a normal-phase chiral HPLC method for the separation of Mirtazapine enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Mirtazapine can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/DEA
Flow Rate1.0 mL/min
DetectionUV 220 nm
Resolution2.84

Description

Class of CompoundsDrug
Analyzing CompoundsMirtazapine

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

Add to cart
Application Analytes:
Mirtazapine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.