HPLC Chiral Separation of Mecoprop Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Mecoprop Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mecoprop.

Mecoprop, also known as methylchlorophenoxypropionic acid and MCPP, is a popular herbicide with the chemical formula C10H11ClO3. It is primarily used to control broadleaf weeds and in combination with other chemically related herbicides including but not limited to 2,4-D, dicamba, and MCPA. It works through mimicking the plant hormone IAA to cause uncontrolled growth, which kills the weed. You can find detailed UV spectra of Mecoprop and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Mecoprop enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Mecoprop can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 250 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/IPA/TFA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution3.35

Description

Class of CompoundsDrug
Analyzing CompoundsMecoprop

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Mecoprop
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.