HPLC Chiral Separation of Hydroxychloroquine on Nonporous Cyralis Columns

HPLC Chiral Method for Analysis of Hydroxychloroquine Enantiomers on Cyralis Screening Kit Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroxychloroquine.

Hydroxychloroquine is a prescription medication with the chemical formula C18H26ClN3O. It is used to treat and prevent acute attacks of malaria in adults and children. It is not used in severe or complicated cases. Besides malaria, Hydroxychloroquine is also used to treat discoid lupus erythematosus (DLE), systemic lupus erythematosus (SLE), and chronic rheumatoid arthritis. It is considered unsafe to take this medication alongside other medications. You can find detailed UV spectra of Hydroxychloroquine and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Hydroxychloroquine enantiomers using SIELC’s Cyralis nonporous chiral columns. The nonporous kit is a collection of AL, CL, AM and CM nonporous phases designed for ultrafast enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis. These nonporous applications are designed to demonstrate baseline resolution of certain racemates even on very short, low-capacity columns.

Hydroxychloroquine can be retained and analyzed using the Cyralis Screening Kit stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of hexane, IPA, and DEA. Detection is performed using UV.

Condition

ColumnCyralis Screening Kit
Mobile PhaseHexane/IPA/DEA – 92/8/0.4%
Flow Rate0.75 mL/min
DetectionUV 330 nm
Resolution1.95

Description

Class of CompoundsDrug
Analyzing CompoundsHydroxychloroquine

Application Column

Cyralis Screening Kit

Select options
Application Analytes:
Hydroxychloroquine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.