HPLC Chiral Separation of Flurbiprofen Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Flurbiprofen Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Flurbiprofen.

Flurbiprofen is a nonsteroidal anti-inflammatory drug with the chemical formula C15H13FO2. It is used primarily as a pre-operative antimiotic and for arthritis and dental pain.

This application describes a normal-phase chiral HPLC method for the separation of Flurbiprofen enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Flurbiprofen can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and TFA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/TFA
Flow Rate1.0 mL/min
DetectionUV 230nm
Resolution1.98

Description

Class of CompoundsDrug
Analyzing CompoundsFlurbiprofen

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Flurbiprofen
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.