HPLC Chiral Separation of Etodolac Enantiomers on Cyralis CM Column

HPLC Method for Analysis of Etodolac on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Etodolac.

Etodolac is a pyrenecarboxylic acid non-steroidal anti-inflammatory drug (NSAID) with the chemical formula C17H21NO3. It is used primarily to manage mild to moderate pain, fever, and inflammation through reducing the levels of prostaglandins.

This application describes a normal-phase chiral HPLC method for the separation of Etodolac enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Etodolac can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/TFA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution4.78

Description

Class of CompoundsDrug
Analyzing CompoundsEtodolac

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Etodolac
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.