HPLC Chiral Separation of Epibatidine on Cyralis CM Column

HPLC Method for Analysis of Epibatidine on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine.

Epibatidine is an alkaloid with the chemical formula C11H13ClN3. It is secreted by the Ecuadorian frog commonly known as Anthony’s Poison Arrow frog from the genus of poison dart frogs, Ameerega. It’s existence in nature is still debated due to limited samples that were collected by John W. Daly, and even fewer samples that were still left when high resolution spectrometry was popularized and used on the compound. As a poison, Epibatidine causes full body numbness which can progress into full body paralysis and respiratory arrest. Due to it’s high toxicity, it is not used as a drug, despite the analgesic effect. You can find detailed UV spectra of Epibatidine and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Epibatidine enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Epibatidine can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/DEA- 99/1/0.02%
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution1.79

Description

Class of CompoundsAlkaloid
Analyzing CompoundsEpibatidine

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Epibatidine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.