HPLC Chiral Separation of Dichlorprop Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Dichlorprop Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dichlorprop.

Dichlorprop is a chlorophenoxy herbicide with the chemical formula C9H8Cl2O3. It is used to kill annual and perennial broadleaf weeds. It is widely used within the United States. It works through increasing cell wall plasticity, biosynthesis of proteins, and the production of ethylene which leads to excessive cell division and therefore damage to vascular tissue. It is acutely toxic, but is a severe eye irritant. You can find detailed UV spectra of Dichlorprop and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Dichlorprop enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Dichlorprop can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 250 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/IPA/TFA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution3.67

Description

Class of CompoundsFungicide
Analyzing CompoundsDichlorprop

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Dichlorprop
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.