HPLC Method for Sucrose octasulfate on Chromni by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucrose octasulfate
Sucrose octasulfate is a chemical compound derived from sucrose, a common sugar with the chemical formula C12H22O35S8. It is characterized by the substitution of eight sulfate groups for hydroxyl groups in the sucrose molecule. This modification significantly changes the properties of the original sugar molecule, making sucrose octasulfate more complex and specialized in its applications.
In terms of its structure, sucrose is a disaccharide composed of glucose and fructose units. When these hydroxyl groups are replaced with sulfate groups in sucrose octasulfate, the molecule becomes highly polar and acidic due to the presence of sulfate groups, which are strong acid residues.
You can find detailed UV spectra of Sucrose octasulfate and information about its various lambda maxima by visiting the following link.
Sucrose octasulfate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid as a buffer. Detection is performed using UV.
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | MeCN/H2O – 75/35 % |
| Buffer | Ammonium Acetate pH 5.0 – 10mM- 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Sugar |
| Analyzing Compounds | Sucrose octasulfate |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
