HPLC MS Method for Analysis of Phosphonoacetic Acid on Chromni Column

HPLC Method for Phosphonoacetic acid on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphonoacetic acid

Phosphonoacetic Acid, also written as PAA, is an organophosphorus compound with the chemical formula C2H5O5P. It is used primarily in research as a targeted inhibitor of DNA polymerase in herpesviruses, cytomegalovirus, and orthopoxyviruses. It is also a structural precursor and model in broad-spectrum antiviral agents.

Phosphonoacetic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an sulfuric acid as a buffer. Detection is performed using UV.

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 75/25%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Class of Compounds
 Acid
Analyzing CompoundsPhosphonoacetic acid

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Phosphonoacetic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.