HPLC Method for Analysis of Ascorbic Acid on Primesep AP Column

HPLC Method for Analysis of Ascorbic Acid on Primesep NC by SIELC Technologies

HPLC Method for Analysis of Ascorbic Acid in on Primesep AP Column

Ascorbic Acid is a vitamin with the molecular formula C6H8O6. Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus. You can find detailed UV spectra of Ascorbic Acid and information about its various lambda maxima by visiting the following link.

Ascorbic Acid can be retained and analyzed using the Primesep AP stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and ammonium acetate. Detection is performed using UV.

Condition

ColumnPrimesep AP, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferAmAc pH 5.0 – 30 mM
Flow Rate0.5 ml/min
DetectionUV 275 nm
Limit of Detection*53.8 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsVitamin
Analyzing CompoundsAscorbic Acid

Application Column

Primesep AP

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ascorbic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.