Homocysteine

Homocysteine structural formula

CAS Number6027-13-0
Molecular FormulaC4H9NO2S
Molecular Weight135.180
InChI KeyFFFHZYDWPBMWHY-VKHMYHEASA-N
LogP-1.30
Synonyms
  • Homocysteine
  • L-Homocysteine
  • 6027-13-0
  • L-Homocysteine
  • (S)-2-Amino-4-mercaptobutanoic acid
  • (S)-Homocysteine
  • 2-Amino-4-mercaptobutyric acid
  • 2-Amino-4-mercapto-L-butyric acid
  • Butanoic acid, 2-amino-4-mercapto-, (S)-
  • Butyric acid, 2-amino-4-mercapto-, L-
  • L-homocisteina
  • L-Homocystein
  • NSC 43117
  • Butanoic acid, 2-amino-4-mercapto-
  • Butyric acid, 2-amino-4-mercapto-
  • EINECS 227-891-0
  • NSC 118376
  • UNII-0LVT1QZ0BA
  • Hcy
  • L-homocysteine zwitterion

Applications:

Separation of Homocysteine on Newcrom R1 HPLC column

February 16, 2018
Separation of Homocysteine on Newcrom C18 HPLC column

Homocysteine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Homocysteine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.