Guanine ribonucleotidyl-(3’-5’)-adenosine

Guanine ribonucleotidyl-(3’-5’)-adenosine structural formula

CAS Number6554-00-3
Molecular FormulaC20H25N10O11P
Molecular Weight612.454
InChI KeyPBWYGOYNGCCLAU-INFSMZHSSA-N
LogP-1.78
Synonyms
  • Guanine ribonucleotidyl-(3'-5')-adenosine
  • (2R,3S,4R,5R)-5-(2-Amino-6-oxo-3,6-dihydro-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate (non-preferred name)
  • 6554-00-3
  • EINECS 229-480-1

Applications:

Separation of Guanine ribonucleotidyl-(3’-5’)-adenosine on Newcrom R1 HPLC column

May 16, 2018
Separation of Guanine ribonucleotidyl-(3’-5’)-adenosine on Newcrom R1 HPLC column

Guanine ribonucleotidyl-(3’-5’)-adenosine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Guanine ribonucleotidyl-(3’-5’)-adenosine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.