Direct Blue 71

Direct Blue 71 structural formula

CAS Number4399-55-7
Molecular FormulaC40H23N7Na4O13S4
Molecular Weight1029.860
InChI KeyQTTDXDAWQMDLOF-PSDAWCJYSA-J
LogP2.28
Synonyms
  • Direct Blue 71
  • Tetrasodium 3-[(E)-{4-[(E)-{4-[(E)-(6-amino-1-hydroxy-3-sulfonatonaphthalen-2-yl)diazenyl]-6-sulfonatonaphthalen-1-yl}diazenyl]naphthalen-1-yl}diazenyl]naphthalene-1,5-disulfonate
  • 1,5-Naphthalenedisulfonic acid, 3-[(E)-2-[4-[(E)-2-[4-[(E)-2-(6-amino-1-hydroxy-3-sulfo-2-naphthalenyl)diazenyl]-6-sulfo-1-naphthalenyl]diazenyl]-1-naphthalenyl]diazenyl]-, sodium salt (1:4)
  • 4399-55-7
  • 1,5-Naphthalenedisulfonic acid, 3-[[4-[[4-[(6-amino-1-hydroxy-3-sulfo-2-naphthalenyl)azo]-6-sulfo-1-naphthalenyl]azo]-1-naphthalenyl]azo]-, tetrasodium salt
  • EINECS 224-531-4
  • C.I. Direct Blue 71
  • 147516-25-4
  • 201749-68-0

Applications:

Separation of Direct Blue 71 on Newcrom R1 HPLC column

February 16, 2018
Separation of Direct Blue 71 on Newcrom C18 HPLC column

Direct Blue 71 can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Direct Blue 71
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.