D&C Red 27

D&C Red 27 structural formula

CAS Number13473-26-2
Molecular FormulaC20H4Br4Cl4O5
Molecular Weight785.660
  • D&C Red 27
  • 2',4',5',7'-Tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxy-
  • 13473-26-2
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxy-
  • 2',4',5',7'-Tetrabrom-4,5,6,7-tetrachlor-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-on
  • 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
  • 2',4',5',7'-Tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxyspiro[isobenzofurane-1(3H),9'-[9H]xanthene]-3-one
  • 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxyspiro[isobenzofuranne-1(3H),9'-[9H]xanthene]-3-one
  • 2',4',5',7'-Tetrabromo-4,5,6,7-tetrachlorofluorescein
  • 2',4',5',7'-tetrabromo-4,5,6,7-tetracloro-3',6'-dihidroxiespiro[isobenzofuran-1(3H),9'-[9H]xanteno]-3-ona
  • 4,5,6,7-Tetrachloro-2',4',5',7'-tetrabromofluorescein
  • Acid Phloxine PB
  • C.I. 45410:1
  • C.I. 45410A
  • C.I. Solvent Red 48
  • D and C Red No. 27
  • D&C Red No. 27
  • Fluorescein, 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-
  • Phloxine BBN Supra
  • Phloxine O
  • Red No. 218
  • Solvent Red 48
  • D & C Red No. 27
  • D&C red no.27
  • EINECS 236-747-6
  • C,I, Solvent Red 48
  • Japan Red 218
  • 119978-11-9
  • 95917-61-6


Separation of D&C Red 27 on Newcrom R1 HPLC column

February 16, 2018
Separation of D&C Red 27 on Newcrom C18 HPLC column

D&C Red 27 can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
D&C Red 27
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.