Bivalirudin

Bivalirudin

CAS Number128270-60-0
Molecular FormulaC98H138N24O33
Molecular Weight2180.317
InChI KeyOIRCOABEOLEUMC-GEJPAHFPSA-N
LogP-0.0816
Synonyms
  • Bivalirudin
  • D-Phenylalanyl-L-prolyl-L-arginyl-L-prolylglycylglycylglycylglycyl-L-asparaginylglycyl-L-alpha-aspartyl-L-phenylalanyl-L-alpha-glutamyl-L-alpha-glutamyl-L-isoleucyl-L-prolyl-L-alpha-glutamyl-L-alpha-glutamyl-L-tyrosyl-L-leucine
  • L-Leucine, D-phenylalanyl-L-prolyl-L-arginyl-L-prolylglycylglycylglycylglycyl-L-asparaginylglycyl-L-alpha-aspartyl-L-phenylalanyl-L-alpha-glutamyl-L-alpha-glutamyl-L-isoleucyl-L-prolyl-L-alpha-glutamyl-L-alpha-glutamyl-L-tyrosyl-
  • 128270-60-0
  • L-Leucine, D-phenylalanyl-L-prolyl-L-arginyl-L-prolylglycylglycylglycylglycyl-L-asparaginylglycyl-L-α-aspartyl-L-phenylalanyl-L-α-glutamyl-L-α-glutamyl-L-isoleucyl-L-prolyl-L-α-glutamyl-L-α-glutamyl-L-tyrosyl-
  • Hirulog
  • Angiomax
  • UNII-TN9BEX005G
  • (2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S,3S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-4-amino-2-[[2-[[2-[[2-[[2-[[(2S)-1-[(2S)-2-[[(2S)-1-[(2R)-2-amino-3-cyclohexylpropanoyl]pyrrolidine-2-carbonyl]amino]-5-carbamimidamidopentanoyl]pyrrolidine-2-carbonyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]-4-oxobutanoyl]amino]acetyl]amino]-4-hydroxy-4-oxobutanoyl]amino]-3-cyclohexylpropanoyl]amino]-5-hydroxy-5-oxopentanoyl]amino]-5-hydroxy-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbonyl]amino]-5-hydroxy-5-oxopentanoyl]amino]-5-hydroxy-5-oxopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoic acidbivalirudina
  • bivalirudine
  • bivalirudinum

Applications:

HPLC Method for Analysis of Bivalirudin and Asp9 – bivalirudin on BIST A+

November 30, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Bivalirudin and Asp9 - bivalirudin on BIST A+
HPLC Method for Analysis of Succinic Acid on BIST A+ Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analyses of Bivalirudin and Asp9 – bivalirudin

Bivalirudin is a short, 21-amino-acid peptide.  It has 2 basic and 6 acidic groups. It is a naturally-occurring anticoagulant.  Asp9-bivalirudin is a process impurity and can be a result of the degradation of the drug bivalirudin.  SIELC’s new BIST™ mode can retain and separate Bivalirudin and its degredant with a TMDAP phosphate buffer, and the respective retention times can be controlled by altering the pH of the buffer.

Condition

ColumnBIST™ A+, 2.1×100 mm, 3µm, 100A
Mobile PhaseMeCN – 30%
BufferTMDAP phosphate – 5 mM  pH 5.0, pH 6.0
Flow Rate0.2 mL/min
DetectionUV 275 nm

Description

Class of CompoundsDrugs, Anticoagulant
Analyzing CompoundsBivalirudin and Asp9 – bivalirudin

Application Column

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Bivalirudin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Bivalirudin on Promix MP colum

February 20, 2016

 

Chr_200

Bivalirudin is a short 21 amino acids peptide.  It has 2 basic and 6 acidic groups. It is a naturally occurred  an anticoagulant.  Asp9 – bivalirudin is a process impurity and can be a result of degradation  of bivalirudin drug.

 

Condition
Column Promix MP, 4.6×250 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 30-60%, 35 min
Buffer Na2HPO4 pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 215 nm

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable, Blood thinners
Analyzing Compounds Bivalirudin

 

Application Column

Promix MP

The Promix family of mixed-mode columns presents an innovative chromatography technology for the efficient resolution of peptides and proteins. This technology hinges on a unique blend of hydrophobic and ionic interactions, facilitated by a novel separation medium: a ligand bonded to a silica support, chemically combined with hydrophobic and ionic functional groups. This phase provides unparalleled selectivity and peak capacity. By independently adjusting the quantities of buffer and organic modifier, a virtually infinite number of separation conditions can be achieved, rendering it suitable for a wide array of biomolecules.

Select options
Application Analytes:
Bivalirudin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.