HPLC Method for Analysis of Bivalirudin and Asp9 – bivalirudin on BIST A+

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Bivalirudin and Asp9 - bivalirudin on BIST A+
HPLC Method for Analysis of Succinic Acid on BIST A+ Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analyses of Bivalirudin and Asp9 – bivalirudin

Bivalirudin is a short, 21-amino-acid peptide.  It has 2 basic and 6 acidic groups. It is a naturally-occurring anticoagulant.  Asp9-bivalirudin is a process impurity and can be a result of the degradation of the drug bivalirudin.  SIELC’s new BIST™ mode can retain and separate Bivalirudin and its degredant with a TMDAP phosphate buffer, and the respective retention times can be controlled by altering the pH of the buffer.


ColumnBIST™ A+, 2.1×100 mm, 3µm, 100A
Mobile PhaseMeCN – 30%
BufferTMDAP phosphate – 5 mM  pH 5.0, pH 6.0
Flow Rate0.2 mL/min
DetectionUV 275 nm


Class of CompoundsDrugs, Anticoagulant
Analyzing CompoundsBivalirudin and Asp9 – bivalirudin

Application Column


BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.