HPLC Method for Bivalirudin on BIST A+ by SIELC Technologies
Bivalirudin is a short, 21-amino-acid peptide with the chemical formula C98H138N24O33. It has 2 basic and 6 acidic groups. It is sold as a direct thrombin inhibitor (DTI), which is used as an anticoagulant.
Asp9-bivalirudin is a process impurity and can be a result of the degradation of the drug bivalirudin. It has the chemical formula C98H137N23O34.
SIELC’s new BIST™ mode can retain and separate Bivalirudin and its degradant with a TMDAP phosphate buffer, and the respective retention times can be controlled by altering the pH of the buffer.
Condition
| Column | BIST A+, 2.1 x 100 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 30% |
| Buffer | TMDAP phosphate – 5 mM pH 5.0, pH 6.0 |
| Flow Rate | 0.2 mL/min |
| Detection | UV 275 nm |
Description
| Class of Compounds | Drugs, Anticoagulant |
| Analyzing Compounds | Bivalirudin |
Application Column
BIST A+
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
BivalirudinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
